The Javid lab is interested in phenotypic resistance of mycobacteria to antibiotics, and in particular, the role of “adaptive mistranslation” in this process.We have previously shown that mycobacteria can modulate specific rates of translational fidelity and that this has important consequences for phenotypic resistance to the first-line anti-tuberculosis agent rifampicin.
Actual projects will be decided near the time but previous Harvard interns have worked on e.g. the following projects and several interns will be co-authors on forthcoming publications:
- A forward genetic screen to identify mycobacterial mutants with high specific rates of mistranslation.
- A suppressor genetic screen to identify specific modulators of GatCAB-mediated regulation of mycobacterial mistranslation.
- Small molecule screen to identify molecules that modulate mycobacterial mistranslation.
- Learning mycobacterial culture and genetics
- Working on semi-independent project under supervision of a senior lab member
- Learning to design experiments, interpret data and present their findings in a semi-formal and formal setting.
- Preference will be given to those with prior wet lab experience.
- Able to withstand, and even enjoy the culture shock of living in Beijing! Please note that Beijing is a moderately polluted city. Please do not apply for this opportunity if living in Beijing for 8-10 weeks will be a problem.
We have always provided housing in international student accommodation at Tsinghua, but this cannot be guaranteed until after the intern has been accepted into the program. There is a very small (<5%) chance that housing may not be available. This hasn’t been an issue to date.
Brief descriptions from the Summer 2017 Fellows:
“This summer, I'm working in the Javid Lab at Tsinghua University in Beijing, focusing on antibiotic resistance in mycobacterial species, non-pathogenic and pathogenic, as it relates to translational fidelity. Somewhat counterintuitively, increases in translational fidelity in mycobacterial species increase the efficacy of antibiotics. Specifically, I am gathering data on the effects of kasugamycin in mycobacterial species. I am also studying whether pre-exposure to an antibiotic can increase their final resistance to that antibiotic, focusing mostly on the antibiotic rifampicin.” – Sherry Gao
“I'm currently researching tuberculosis and antibiotic resistance. We are conducting assays of multiple first and second-line drugs used to treat TB and using two color flow cytometry to observe how TB growth and vitality are affected by different concentrations of these antibiotics. Our ultimate aim is to identify persistent populations and conduct genetic analysis on them, which will hopefully provide greater insight for clinical treatment of TB, multi-drug resistant TB, and extremely dug resistant TB.” –Montita Sowapark